Part of I-41 in Oshkosh was closed for five hours Monday night, into Tuesday morning.
A tractor and car collided around 7:45 p.m. on Monday near W. 20th Ave.
A 44 year-old woman from Eden hit the back of a John Deere tractor.
She was taken to the hospital with non-life threatening injuries.
The cause of the crash remains under investigation.
All lanes were reopened shortly before 2:00 a.m.